[CellMosaic] Antibody Mertansine (DM1) Conjugation Kit

[CellMosaic] Products

[CellMosaic] Antibody Mertansine (DM1) Conjugation Kit

 

Product Description:

CellMosaic는 항체의 초기 단계 screening을 위해 SMCC (succinimidyl trans-4-(maleimidylmethyl)cyclohexane-1-carboxylate) linker를 사용한 mertansine (DM1)의 antibody (IgG type) 라벨링을 위해 이 키트를 설계했습니다.

이 키트에는 표면 아민을 통해 항체에 순차적으로 결합할 수 있는 SMCC와 DM1이 포함되어 있습니다. 제품은 반응하지 않은 약물을 제거하기 위해 정제됩니다. 

Standard Kit Configuration for CM11410 (Default is 1-3 mg and 1 Reaction):
 

Antibody Amount per Reaction	Number of Reactions in Kit	Catalog Number
0.1 mg	1 or 3	CM11410.01x1 or CM11410.01x3
1-3 mg	1 or 3	CM11410x1 or CM11410x3

Drug Information:

• Name: Mertansine (DM1) 
• CAS number: 139504-50-0
• Mechanism of action: Inhibits cell division by blocking the polymerisation of tubulin

Requirement for Antibody:

• Preferably >90% pure by gel electrophoresis
• Total amount: 1-3 mg or 0.1 mg of protein content as measured by UV. 

Key Features of this PerKit™:

• Offers a simple and easy way to label IgG with DM1 with minimum exposure to the toxin
• Stable linkage 
• Fast and easy preparation: 6 h preparation and <2 h hands-on time
• All reagents and supplies included for preparation and purification
• Average 2-4 DM1 labeling per antibody
• Less than 5% of antibody aggregation and >99% of conjugated products by SEC (size-exclusion chromatography)
• Options to choose tailored services at CellMosaic® after conjugation

 

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